What is Peptide Conjugation?

Peptides are short chains of amino acids that play essential roles in biological processes and have emerged as versatile molecules for various applications. However, the inherent limitations of peptides, such as poor stability and rapid degradation, often hinder their practical utility. Peptide conjugation, through the attachment of functional groups or molecules, offers a solution to enhance the properties and expand the applications of peptides. Peptide conjugation is covalently linking a peptide to another molecule or entity, such as a protein, drug, or fluorescent probe. This can be achieved through various chemical reactions, including amide bond formation, thiol-maleimide coupling, and click chemistry.

Advantages of Peptide Conjugation

• Increased specificity
• Enhanced stability
• Targeted delivery
• Versatility

Application of Peptide Conjugation

• Improving the efficacy and specificity of drugs
• Peptide-Based Imaging Probes

Creating imaging probes for a variety of applications, such as in vivo imaging, fluorescent microscopy, and magnetic resonance imaging (MRI). Peptides can be conjugated to imaging agents, such as fluorophores, radiolabels, or magnetic nanoparticles, to develop highly specific imaging probes.

• Diagnostic Assays

Developing diagnostic assays for detecting specific biomarkers or diseases. Peptide conjugation can enable the detection and imaging of specific targets with high sensitivity and specificity.

• Modifying the properties of biomaterials for tissue engineering or drug delivery applications.
• Developing peptide-based vaccines
• Developing biosensors for detecting analytes in complex biological samples.

Peptides can be immobilized onto sensor surfaces, either directly or through conjugation with recognition elements (e.g., antibodies, aptamers), to enable specific and sensitive detection. Peptide-based biosensors offer advantages such as rapid response, high selectivity, and potential for miniaturization, making them valuable tools in clinical diagnostics, environmental monitoring, and food safety.

• Drug Delivery Systems

By improving the pharmacokinetics, stability, and targeting capabilities of therapeutic agents.

• Prodrug Strategies

Peptide conjugation can be employed to design prodrugs, which are inactive or less active forms of drugs that undergo enzymatic or chemical activation at the target site.

• Materials Science

Peptide conjugation has opened up new possibilities in materials science, enabling the fabrication of functional materials with diverse applications.

Main Advantages

• Highly stable conjugate
• Muti-type conjugations available

Peptide conjugation through N-terminal amine, C-terminal acid, Cys thiol, or other functional groups are available.

• Save time and cost
• Purification

Depending on the type of molecule used for conjugation, normally low molecular weight contaminants will be eliminated by SEC chromatography or HPLC.

• Quality Control

Gel electrophoresis, MALDI MS (except for KLH), or other appropriate analysis.

Creative Peptides has developed a reliable method for custom peptide conjugation, and we will provide custom peptide conjugation services to global customers with excellent technology. Every step of peptide synthesis is subject to Creative Peptides' stringent quality control. The experienced experts and advanced instruments in Creative Peptides can meet all your needs for peptide conjugation. We will provide you with a full range of synthesis and modification services. As a partner in your research, we look forward to deeper cooperation. Please contact us!

Source: https://www.creative-peptides.com/services/custom-conjugation-service.html