RNA Pull Down Assay for Plant: A Powerful Tool to Study RNA-Protein Interactions
RNA pull-down assay is a useful tool for researching RNA-protein interactions in plants. The idea is to separate proteins that attach themselves to RNA by using RNA with biotin tagging as bait. First, attach the biotin-tagged RNA to streptavidin beads, next, add a cell lysate or protein extract to the mix. The beads will attract and pull the RNA-binding proteins, and the RNA-protein complexes...
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